Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay

Masanori KANAZAWA1)2), Shinobu SATO3), Keiichi OHTSUKA1) and Shigeori TAKENAKA1)

1) Department of Applied Chemistry, Kyushu Institute of Technology 2) Laboratory Water Division, Nihon Millipore K. K. 3) Department of Bioscience and Bioinformatics, Kyushu Institute of Technology

(Received September 6, 2007) (Accepted October 15, 2007)

Messenger RNA (mRNA) poly(A)+RNA (from mouse kidney) was immobilized on a N-hydroxysuccinimide(NHS)-activated carboxylic acid modified electrode prepared by the treatment of a gold electrode with 3,3′-dithiodipropionic acid, followed by NHS and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). An electrochemical measurement using this mRNA electrode was carried out in an electrolyte containing ferrocenylnaphthalene diimide (1), and showed an electrochemical signal based on 1 concentrated on immobilized mRNA. After treating this electrode with water containing varied amounts of ribonuclease A (RNase A), the current peak based on 1 decreased with increasing in the amount of RNase A with a linear correlation in the range of 0.2 - 10 pg of RNase A.

Anal. Sci., Vol. 23, p.1415, (2007)